Isolation and Identification of a Chitin Deacetylase-Producing Brevibacillus borstelensis Strain
Abstract
protection. Chitin deacetylase (CDA) can catalyze this conversion process. Compared to the traditional concentrated alkali thermochemical
method, enzymatic catalysis offers advantages such as milder conditions, controllable deacetylation sites, environmental friendliness, and
preservation of the chitin backbone structure. However, the current availability of high-yielding CDA strain resources is limited, and research
on bacterial-derived CDA, in particular, remains relatively scarce, hindering the green biomanufacturing of chitosan. In this study, a marine
bacterium producing CDA was isolated from sediment in the mangrove area of Zhanjiang, Guangdong Province, China.. Based on morphological observation and 16S rDNA sequence analysis, the strain was identified as Brevibacillus borstelensis. This research provides a new
microbial resource for chitin deacetylase development and a methodological basis for the enzymatic preparation of chitosan.
Keywords
Full Text:
PDFReferences
[1] Maniyan V, Panda S K. Biotechnological Advances in Chitin and Chitosan-Based Biocomposites: Challenges and Opportunities[J]. Biotechnological Advances in Chitin and Chitosan based Biocomposites, 2025: 1-24.
[2] Thambiliyagodage C, Jayanetti M, Mendis A, et al. Recent advances in chitosan-based applicationsa review[J]. Materials, 2023,
16(5): 2073.
[3] Zhang M, Zhang F, Li C, et al. Application of chitosan and its derivative polymers in clinical medicine and agriculture[J]. Polymers,
2022, 14(5): 958.
[4] Chicea D, Nicolae-Maranciuc A. A review of chitosan-based materials for biomedical, food, and water treatment applications[J]. Materials, 2024, 17(23): 5770.
[5] Huang Z, Lv X, Sun G, et al. Chitin deacetylase: From molecular structure to practical applications[J]. Systems Microbiology and Biomanufacturing, 2022, 2(2): 271-284.
[6] Raghavan N, Pathan E K. A comprehensive account of fungal chitin deacetylases: Aspects and prospects[J]. International Journal of
Biological Macromolecules, 2025: 142705.
[7] Amer O A, Ali S S, Azab M, et al. Exploring new marine bacterial species, Alcaligenes faecalis Alca F2018 valued for bioconversion of
shrimp chitin to chitosan for concomitant biotechnological applications[J]. International Journal of Biological Macromolecules, 2022,
196: 35-45.
[8] Zhang Y, Luo X, Yin L, et al. Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan
preparation[J]. Frontiers in Bioengineering and Biotechnology, 2023, 11: 1183333.
[9] Liang Y, Yan L, Tan M, et al. Isolation, characterization, and genome sequencing of a novel chitin deacetylase producing Bacillus aryabhattai TCI-16[J]. Frontiers in Microbiology, 2022, 13: 999639.
[10] Miao D S, Ding Z W, Li T, et al. Screening of chitin deacetylase-producing Lysinibacillus macroides ZCGT05 and study on its enzymatic properties[J]. 2022.
[11] Xu Y, Chen L, Yang L, et al. Chitinibacter mangrovi sp. nov., a Novel Chitin-Degrading Bacterium Isolated from Mangrove
Sediment[J]. Current Microbiology, 2025, 82(7): 299.
DOI: http://dx.doi.org/10.70711/eph.v3i1.8587
Refbacks
- There are currently no refbacks.
