Molecular Diagnostic Applications of the CRISPR/Cas System
Abstract
safety, and environmental monitoring, offering significant value in pathogen surveillance, treatment guidance, and disease prevention. In recent years, CRISPR- and CRISPR/Cas-based methods have emerged as a novel nucleic acid detection platform. These approaches are regarded as next-generation diagnostic tools due to their high specificity, sensitivity, and user-friendly features, with promising potential for diverse
applications.
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[1] Roers A, Hiller B, Hornung V. Recognition of endogenous nucleic acids by the innate immune system [J]. Immunity, 2016, 44(4): 739-
754.
[2] Li Y, Li S, Wang J, et al. CRISPR/Cas systems towards next-generation biosensing [J]. Trends in biotechnology, 2019, 37(7): 730-743.
[3] Pardee K, Green AA, Takahashi MK, et al. Rapid, low-cost detection of Zika virus using programmable biomolecular components [J].
Cell, 2016, 165(5): 1255-1266.
[4] Huang M, Zhou X, Wang H, et al. Clustered regularly interspaced short palindromic repeats/Cas9 triggered isothermal amplification for
site-specific nucleic acid detection [J]. Analytical chemistry, 2018, 90(3): 2193-2200.
[5] Wang T, Liu Y, Sun HH, et al. An RNA-guided Cas9 nickase-based method for universal isothermal DNA amplification [J]. Angewandte
Chemie, 2019, 131(16): 5436-5440.
[6] Dubey AK, Kumar Gupta V, Kujawska M, et al. Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and
treatment of infectious diseases [J]. Journal of Nanostructure in Chemistry, 2022, 12(5): 833-864.
[7] Gootenberg JS, Abudayyeh OO, Lee JW, et al. Nucleic acid detection with CRISPR-Cas13a/C2c2 [J]. Science, 2017, 356(6336): 438-
442.
[8] Gootenberg JS, Abudayyeh OO, Kellner MJ, et al. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and
Csm6 [J]. Science, 2018, 360(6387): 439-444.
[9] Kaminski MM, Abudayyeh OO, Gootenberg JS, et al. CRISPR-based diagnostics [J]. Nature Biomedical Engineering, 2021, 5(7): 643-
656.
[10] Li S-Y, Cheng Q-X, Wang J-M, et al. CRISPR-Cas12a-assisted nucleic acid detection [J]. Cell discovery, 2018, 4(1): 20.
[11] Chen JS, Ma E, Harrington LB, et al. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity [J]. Science, 2018, 360(6387): 436-439.
[12] Broughton JP, Deng X, Yu G, et al. CRISPRCas12-based detection of SARS-CoV-2 [J]. Nature biotechnology, 2020, 38(7): 870-874.
DOI: http://dx.doi.org/10.70711/pmr.v2i6.7254
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