Ziyin Zhong; Xiaoyan Zou*; Shengqi Jiang; Ruolan Wu; Wenxia Lei; Jiajia Xiao

doi:10.70711/pmr.v3i6.9140

Exploring the Multi-Target Mechanisms of Andrographolide in the Treatment of Sjren's Syndrome Based on Network Pharmacology and Molecular Docking

Ziyin Zhong, Xiaoyan Zou*, Shengqi Jiang, Ruolan Wu, Wenxia Lei, Jiajia Xiao

Abstract

Sjrens syndrome (SS) is a chronic autoimmune disease with limited treatment options. Andrographolide, a bioactive compound
from Andrographis paniculata, possesses anti-inflammatory properties, but its multi-target mechanisms in SS remain unclear. This study used
network pharmacology and molecular docking to investigate potential targets and pathways of andrographolide in SS treatment. Androgra
pholide-related targets and SS-associated genes were retrieved from multiple databases and the GSE66795 dataset. Protein-protein interaction
analysis identified hub targets including TNF, IL-6, IL-1?, STAT3, and NFKB1 . GO and KEGG enrichment revealed involvement in inflam
matory response, apoptosis regulation, and key pathways such as TNF signaling, NF-?B signaling, and Th17 differentiation. Molecular dock
ing confirmed strong binding affinities between andrographolide and core targets. These findings suggest andrographolide exerts therapeutic
effects in SS through multi-target modulation of inflammatory and immune pathways, providing a scientific basis for further research and
clinical application.

Keywords

Full Text:

PDF

Included Database

References

[1] Horiba, Y., Ishizawa, K., Takasaki, K., Miura, J., & Babazono, T. (2022). Effect of depression on progression to end-stage renal disease

or pre-end-stage renal disease death in advanced diabetic nephropathy:A prospective cohort study of the Diabetes Study from the Center

of Tokyo Women's Medical University. Journal of Diabetes Investigation, 13, 94101.

[2] Huang, S., Jin,Y., Zhang, L., Zhou,Y., Chen, N., & Wang, W. (2024). PPAR gamma and PGC-1alpha activators protect against diabetic

nephropathy by suppressing the inflammation and NF-?B activation.Nephrology (Carlton), 29, 858872.

[3] Cho, C. H., Roh, K. H., Lim, N. Y., Park, S. J., Park, S., & Kim, H. W. (2022). Role of the JAK/STAT pathway in a streptozotocin

induced diabetic retinopathy mouse model. Graefes Archive for Clinical and Experimental Ophthalmology, 260, 35533563.

[4] Ding, Y., Chen, L., Wu, W., Yang, J., Yang, Z., & Liu, S. (2017).Andrographolide inhibits influenza A virus-induced inflammation in a

murine model through NF-?B and JAK-STAT signaling pathway. Microbes and Infection, 19, 605615.

[5] Dixit, N., Motwani, H., Patel, S. K., Rawal, R. M., & Solanki, H. A. (2024). Decoding the mechanism of andrographolide to combat

hepatocellular carcinoma: A network pharmacology integrated molecular docking and dynamics approach. Journal of Biomolecular

Structure and Dynamics, 42, 1023710255.

[6] Hall, S. E., Upton, R. M. O., McLaughlin, E. A., & Sutherland, J. M. (2018). Phosphoinositide 3-kinase/protein kinase B (PI3K/AKT)

and Janus kinase/signal transducer and activator of transcription (JAK/STAT) follicular signaling is conserved in the mare ovary. Repro

duction, Fertility and Development, 30, 624633.

DOI: http://dx.doi.org/10.70711/pmr.v3i6.9140

Refbacks

There are currently no refbacks.