Establishment and Validation of Multiplex PCR Assay for Simultaneous Identification of Four Common Foodborne Pathoge
Abstract
Listeria monocytogenes and Escherichia coli O157: H7. It had good specificity with no cross-reaction to non-target bacteria. The detection
limits were very low for pure cultures and also low for artificially contaminated food after pre-enrichment. The assay showed excellent repeatability. For a large number of commercial food samples, its consistency with the national standard method was very high. The detection time
was greatly shortened to a short period, with high efficiency, low cost and strong anti-interference.
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DOI: http://dx.doi.org/10.70711/frim.v4i7.9865
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